Optical configuration of the confocal optical probe. An optical fiber serves as a pinhole and is scanning in X- and Y-direction (Y-axis is pointing out of the page). The collimating lens (L1) is at a fixed position, while the objective lens (L2) is scanning in Z-direction.
The significance of this study is to improve confocal imaging technology by combining pneumatic actuation (for Z-axis) and piezoelectric actuations (for X-Y axes).
The pneumatic actuator has a feedback control mechanism and has a displacement range of 200 um and high resolution (1.5 um).
To demonstrate the functionality of the system, paper fiber and onion epidermis were used to obtain confocal images.
(a) Schematic diagram of the imaging system; Full assembly of the probe, actuators and lenses: (b) Top-view of the scanner system. (c) Close-up view of the pneumatic actuator.
Images stacks of a paper fiber sample to form a 3D image
The X-Y optical resolution of the images is 1.4 um and the field-of-view is 100 um × 100 um with a framerate of 1.25 fps. It was observed that using the PI controller would improve the quality of images.